Optimized cryopreservation - tissue preserved, including viable cells
Cryopreservation preserves tissue in dimethyl sulfoxide and cryoprotectant solution at a controlled cooling rate. Tissue then has a long shelf-life but must be stored and shipped at below -75°C, therefore requiring access to specialized freezing equipment.
Cryopreservation preserves tissue integrity, growth factors and the ECM, and retains the native cells of the placenta in a viable form.
H&E (hematoxylin and eosin) staining show tissue morphology and MT (Masson’s trichrome) staining shows collagen distribution.
Cell Viability
Staining for Live and Dead Cells
Green = Live cells
Red = Dead cells
Compared with devitalized dehydrated tissue, cryopreserved tissue has demonstrated significant improvement in wound closure functions.
- Cooke M, Tan EK, Mandrycky C, He H, O’Connel J, Tsend SC. Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue. J Wound Care. 2014;23(10):465–474.
- Dhall S, Sathyamoorthy M. Kuang JQ, et al. Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage. PLoS One. 2018;13(10):e0204060.
- Johnson A, Gyurdieva A, Dhall S, Danilkovitch A, Duan-Arnold L. Understanding the impact of preservation methods on the integrity and functionality of placental allografts. Ann Plast Surg. 2017;79(2):203–213.
- Johnson EL, Marshall JT, Michael GM. A comparative outcomes analysis evaluating clinical effectiveness in two different human placental membrane products for wound management. Wound Repair Regen. 2017;25(1):145–149.
- Reyzelman AM, Vartivarian M, Danilkovitch A, Saunders MC. A prospective, single-center, open-label case series evaluating the clinical outcomes of lyopreserved placental membrane containing viable cells in the treatment of chronic wounds. Wounds. 2019;31(4):97-102.